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300 MHz 1H/31P(13C) probe |
1. PURPOSE
This static variable temperature probe was built to probe alignment of lipid bilayers
in oriented membrane protein samples, by looking at their 31P spectra.
Such measurement finds if the sample is worth studying with 1H/15N probe
at higher field spectrometer. Put together, these two probes provide facility at NHMFL to
study membrane proteins. The low-frequency channel can also be retuned by user to look at 13C signal by
swapping couple of elements in the RF circuit. Tiny concentrations of 15N-labeled protein dictate use
of large sample volume coils in both probes. Default RF coil in this instrument is 14 mm in diameter.
The probe is equipped with variable temperature capability, able to mantain sample within common biological temperature range.
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RF coils can be swapped with ease |
One of best features of this probe is its versatility when it comes to RF components.
The RF sample coil is easily detachable by unscrewing it from top of the probe and can be replaced
by coil of different size and geometry (e.g. square solenoid). Other circuit elements such as
isolation traps or capacitor add-ons can also be changed by a facility user without resorting to soldering.
2. PROBE FEATURES
- Double-tuned 1H/31P (or 13C)
- User-exchangeable RF coils.
- Largest coil size is 14 mm (default)
- User-exchangeable traps and add-on capacitors.
- Sample temperature range: -40°C ... +100°C
- Body air
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